Table 2. Preparative HPLC conditions

Time (min)				Solvent A 3 Distilled water 99.9%				Solvent B Acetonitrile 99.9%
Initial				100				0
20				90				10
25				40				60
30				20				80
45				0				100
50				0				100
55				100				0
60				100				0
Flow			5 mL/min			Temperature			30°C
Wavelength			220 nm			Injection volume			2,000 μL